Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 2 Cytotoxicity of PTPRO in HFL1. (A) Transfection with pcDNA-PTPRO or shPTPRO increased or decreased PTPRO expression, respectively. (B) Overexpression of PTPRO reduced cell viability of HFL1, while silencing of PTPRO did not affect cell viability. (C) Overexpression of PTPRO promoted cell apoptosis of HFL1, while silencing of PTPRO did not affect cell apoptosis. *p < 0.05, **p < 0.01.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Transfection, Expressing, Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 1 Lipopolysaccharide induced the elevation of PTPRO in HFL1. (A) Incubation with lipopolysaccharide reduced cell viability of HFL1 in a dose-dependent way. (B) Incubation with lipopolysaccharide enhanced protein expression of PTPRO in HFL1 in a dose-dependent way. *p < 0.05, ***p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Incubation, Expressing

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 3 PTPRO contributed to lipopolysaccharide-induced apoptosis in HFL1. (A) Transfection with pcDNA-PTPRO reduced the cell viability of lipopolysaccharide-induced HFL1, while transfection with shPTPRO enhanced cell viability. (B) Overexpression of PTPRO increased cell apoptosis of lipopolysaccharide-induced HFL1, while transfection with shPTPRO inhibited cell apoptosis. (C) The apoptotic ratio of lipopolysaccharide-induced HFL1 that was transfected with pcDNA-PTPRO or shPTPRO. #p < 0.05; ##,^^p < 0.01; ***,###p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Transfection, Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 4 PTPRO contributed to lipopolysaccharide-induced inflammation in HFL1. (A) mRNA expressions of TNF-α, IL-1β, and IL-18 in lipopolysaccharide-induced HFL1 were enhanced by PTPRO overexpression, while they were reduced by PTPRO silence. (B) Protein expressions of TNF-α, IL-1β, and IL-18 in lipopolysaccharide-induced HFL1 were enhanced by PTPRO overexpression, while they were reduced by PTPRO silence.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 5 PTPRO contributed to lipopolysaccharide-induced activation of TLR4/NF-κB in HFL1. (A) Protein expressions of TLR4, p-p65, and p-IκBα in lipopolysaccharide-induced HFL1 were increased by PTPRO overexpression, while they were decreased by PTPRO silence. #,^p < 0.05; ^^p < 0.01, ***,###p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Activation Assay, Over Expression